Journal: Frontiers in Immunology

Article Title: Regulatory Dendritic Cells Induced by Bendamustine Are Associated With Enhanced Flt3 Expression and Alloreactive T-Cell Death

doi: 10.3389/fimmu.2021.699128

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: Anti-human BDCA3 APC-Vio770 , REA774 , Miltenyi Biotec.

Techniques: Cytometry

Journal: Frontiers in Immunology

Article Title: Regulatory Dendritic Cells Induced by Bendamustine Are Associated With Enhanced Flt3 Expression and Alloreactive T-Cell Death

doi: 10.3389/fimmu.2021.699128

Figure Lengend Snippet: Human DCs exhibit concentration-dependent increase in Flt3 expression following BEN exposure and decreased pSTAT3. Human monocyte-derived DCs (moDCs) were generated following brief exposure to BEN and characterized by flow cytometry. Data shown is pooled from 9 independent experiments (n=5-9). (A) Mean Flt3 MFI among human moDCs shown with SEM. (B) Representative histogram of Flt3 expression on BEN-exposed moDCs from a single individual. (C) Mean pSTAT3 MFI normalized to percent of control (0μM) shown with median. (D) Mean percent of plasmacytoid DCs (Lineage - CD11c + BDCA4 + ) shown with SEM. (E) Mean percent of cDC1 (Lineage - CD11c + BDCA4 - BDCA3 + ) shown with SEM. (F) Mean percent cDC2 (Lineage - CD11c + BDCA4 - BDCA1 + ) shown with SEM. One-way ANOVA and Dunnett’s multiple comparisons test were used to determine significance among groups. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Anti-human BDCA3 APC-Vio770 , REA774 , Miltenyi Biotec.

Techniques: Concentration Assay, Expressing, Derivative Assay, Generated, Flow Cytometry, Control

Journal: PLoS ONE

Article Title: Allergen-Specific Immunotherapy Alters the Frequency, as well as the FcR and CLR Expression Profiles of Human Dendritic Cell Subsets

doi: 10.1371/journal.pone.0148838

Figure Lengend Snippet: (A). Specific DC subsets were identified as HLA-DR positive, lineage negative cells that expressed either CD141, CD1c, CD16 or CD123, using flow cytometric analysis of viable cells in the FSC-SSC gate. (B). Flow cytometric analysis was performed on non-allergic controls (n-a) (n = 6) as well as on AIT subjects (n = 7) before treatment (t0), as well as after 8 weeks (t8w) and 1 year (t1y), to determine the frequencies of CD141 + , CD1c + , CD123 + and CD16 + DC subsets. Plots show each donor value as well as mean values and SD variations. * = p<0.05, Friedman’s test followed by a Dunn's multiple comparison test.

Article Snippet: Antibodies used included rabbit anti-mouse-PE, CD14-PE, CD19-PE, CD19-FITC (Dako Cytomation, Glostrup, Denmark), goat anti-rabbit-FITC, CD123-biotin, CD123-PE, CD16-PeCy7, CD3-PE, CD3-FITC, CD32-FITC, SA-APCCy7 (BD Biosciences, San Jose, CA), CD1c-PE, CD141-APC, CD141-biotin (Miltenyi Biotec, Bergisch Gladbach, Germany), HLA-DR-PerCp-Cy5.5 (Biolegend, San Diego, CA), CD14-FITC, CD3-APC, CD14-APC and CD19-APC (Invitrogen, Carlsbad, CA), CD301-Alexa fluor 488 (Dendritics, Lyon, France), CD206-PE, CD207/Langerin-PE (Beckman Coulter, Brea, CA), CD205-FITC, FcεRI-biotin (eBioscience, San Diego, CA), Dectin-1/CLEC7A-FITC (AbD Serotec, Kidlington, UK), Dectin-2, DCIR/CLECSF6, DNGR1, CD64-PE (R&D Systems, Minneapolis, MN) and CD280/C-type mannose receptor 2 (MRC2), CD209 (Abcam, Cambridge, UK).

Techniques: Comparison

Journal: PLoS ONE

Article Title: Allergen-Specific Immunotherapy Alters the Frequency, as well as the FcR and CLR Expression Profiles of Human Dendritic Cell Subsets

doi: 10.1371/journal.pone.0148838

Figure Lengend Snippet: FcR expression based on flow cytometric analysis on cells from non-allergic controls (n-a) (n = 6) and AIT donors at t0, t8w and t1y (n = 7, with the exception of FcεRI on CD141 + DCs (n = 2) and CD1c + DCs (n = 6)). Plots show each donor value as well as mean values and SD variations. * = p<0.05; ** = p<0.01, (Friedman’s test followed by a Dunn's multiple comparison test. FcRs altered significantly during AIT are framed. FcεRI on CD141 + DCs not tested for significance, too few AIT samples).

Article Snippet: Antibodies used included rabbit anti-mouse-PE, CD14-PE, CD19-PE, CD19-FITC (Dako Cytomation, Glostrup, Denmark), goat anti-rabbit-FITC, CD123-biotin, CD123-PE, CD16-PeCy7, CD3-PE, CD3-FITC, CD32-FITC, SA-APCCy7 (BD Biosciences, San Jose, CA), CD1c-PE, CD141-APC, CD141-biotin (Miltenyi Biotec, Bergisch Gladbach, Germany), HLA-DR-PerCp-Cy5.5 (Biolegend, San Diego, CA), CD14-FITC, CD3-APC, CD14-APC and CD19-APC (Invitrogen, Carlsbad, CA), CD301-Alexa fluor 488 (Dendritics, Lyon, France), CD206-PE, CD207/Langerin-PE (Beckman Coulter, Brea, CA), CD205-FITC, FcεRI-biotin (eBioscience, San Diego, CA), Dectin-1/CLEC7A-FITC (AbD Serotec, Kidlington, UK), Dectin-2, DCIR/CLECSF6, DNGR1, CD64-PE (R&D Systems, Minneapolis, MN) and CD280/C-type mannose receptor 2 (MRC2), CD209 (Abcam, Cambridge, UK).

Techniques: Expressing, Comparison

Journal: PLoS ONE

Article Title: Allergen-Specific Immunotherapy Alters the Frequency, as well as the FcR and CLR Expression Profiles of Human Dendritic Cell Subsets

doi: 10.1371/journal.pone.0148838

Figure Lengend Snippet: Flow cytometric analysis was performed on CD141 + DCs from non-allergic donors (n = 6), as well as allergic donors (n = 5) at t0, t8w and t1y. Plots show each donor value as well as mean values and SD variations. Statistical analysis of AIT donors compared to non-allergic donors was performed with a Kruskal-Wallis test followed by a Dunn's multiple comparison test. * = p<0.05. ns-non-significant; w-weeks; y-years.

Article Snippet: Antibodies used included rabbit anti-mouse-PE, CD14-PE, CD19-PE, CD19-FITC (Dako Cytomation, Glostrup, Denmark), goat anti-rabbit-FITC, CD123-biotin, CD123-PE, CD16-PeCy7, CD3-PE, CD3-FITC, CD32-FITC, SA-APCCy7 (BD Biosciences, San Jose, CA), CD1c-PE, CD141-APC, CD141-biotin (Miltenyi Biotec, Bergisch Gladbach, Germany), HLA-DR-PerCp-Cy5.5 (Biolegend, San Diego, CA), CD14-FITC, CD3-APC, CD14-APC and CD19-APC (Invitrogen, Carlsbad, CA), CD301-Alexa fluor 488 (Dendritics, Lyon, France), CD206-PE, CD207/Langerin-PE (Beckman Coulter, Brea, CA), CD205-FITC, FcεRI-biotin (eBioscience, San Diego, CA), Dectin-1/CLEC7A-FITC (AbD Serotec, Kidlington, UK), Dectin-2, DCIR/CLECSF6, DNGR1, CD64-PE (R&D Systems, Minneapolis, MN) and CD280/C-type mannose receptor 2 (MRC2), CD209 (Abcam, Cambridge, UK).

Techniques: Comparison

Journal: Immunity

Article Title: Transcriptional and Functional Analysis of CD1c + Human Dendritic Cells Identifies a CD163 + Subset Priming CD8 + CD103 + T Cells

doi: 10.1016/j.immuni.2020.06.002

Figure Lengend Snippet:

Article Snippet: VioBlue anti-human CD141 antibody (clone AD5-14H12) , Miltenyi , Cat# 130-113-882; RRID: AB_2726374.

Techniques: Purification, Virus, In Vitro, Recombinant, Isolation, Enzyme-linked Immunosorbent Assay, Software